Non-Interfering Protein Assay Kit With Albumin Standard - 1 Kit

Non-Interfering Protein AssaySimple to follow protein assay overcomes interference of agents commonly present in protein solutions and shows no protein-to-protein variation.Non-Interfering Protein Assay has been extensively tested to work in the presence of reducing agents, chelating agents, detergents, amines, and numerous other agents and is not affected even if the protein is in strong extraction buffers.Proprietary Universal Protein Precipitating Agent , UPPA, removes interfering agents from protein solutions.When mixed with protein solution, UPPA rapidly precipitates protein in the sample tube and this allows removal of interfering agents present in the protein solution.Mechanism:Assay is based on the specific binding of copper ions to the peptide back bone of protein.Protein is mixed with an alkaline copper solution and the unbound copper is quantitatively determined by the degree of color change.As the concentration of protein increases, the concentration of unbound copper decreases.The color, therefore, is inversely related to the amount of protein and allows the Non-Interfering Protein Assay to eliminate protein-to-protein variations.Since any protein modifications (glycosylation, etc.) or the presence of normally interfering compounds do not affect this protein assay, you get reliable results everytime from different samples.Specifications:Removing interfering agents from protein solution and measuring unbound copper in the protein solution results in a highly reliable determination of protein concentration using automated, semi-automated, or manual spectrophotometric techniques of as little as 0.5µg protein with a linear response for 0.5-60µg protein per assay.Simple protocol yields excellent results in as little as 15 minutes.Components: 2ml assay tubes, disposable cuvettes and albumin protein standard.For less than 1.5ml cuvettes, scale ALL reagents down.500 assays. Application: Method: Species: Storage:2-8°C