NF-1 Transcription Factor NoShift II Assay Kit - 96 rxn

The Nuclear factor-1 (NF-1) family of transcription factors are encoded by the genes NF-1A, NF-1B, NF-1C and NF-1X and feature a highly conserved N-terminal DNA binding region and a variable C-terminal transcriptional activation or repression domain. NF-1 binding controls the expression of many target genes, including insulin, TGF-b, nucleotide, steroid hormone and vitamin synthetic genes, TNFa and FSH. NF-1 transcripts can be differentially spliced and the resultant protein variants homo- and heterodimerize, allowing for multiple potential transcriptional activation and repression activities. TheNF-1 transcription factors can be rapidly detected in a convenient 96-well format by the NoShift⢠II Transcription Factor Assay System. This sensitive, non-radioactive assay can be completed within four hours and is a superior alternative to standard electromobility shift assays (EMSA)