PBlue Phosphate Assay Kit - 500 assays

The PBlue Phosphate Assay Kit is based on an innovative formulation of the malachite green dye. The PBlue reagent forms a blue coloured complex with free orthophosphate. The rapid colour formation from the reaction can be conveniently measured on a spectrophotometer (600 - 660nm) or on a plate reader. This single reagent âmix-and-measureâ colourimetric assay has been optimised to offer superior sensitivity and prolonged shelf life. The assay is simple and fast (quantitation of free phosphate within 30 minutes), involving a single addition step for phosphate determination. Applications include phosphatase assays, lipase assays, nucleoside triphosphatase assays and high-throughput screening for phosphatase inhibitors (drug discovery). Application:1. Phosphatase Assays: liberation of phosphate from peptide, protein or small molecule substrate.2. Lipase Assays: liberation of phosphate from phospholipids.3. Nucleoside Triphosphatase Assays: liberation of phosphate from nucleoside triphosphates (ATP, GTP, TTP, CTP etc).Quantitation of Phosphate in phospholipids, proteins and DNAs, etc.4. Drug Discovery: high-throughput screen for phosphatase inhibitors. Method: Species: Storage:Stored at 4°C. Stability:Stable for 12 months from production. Sensitivity: Time: