Rat Alpha-1 Acid Glycoprotein ELISA Kit 1 Kit (96 Wells) - 1 Kit

The principle of the double antibody sandwich ELISA is represented in Figure 1.  In this assay the Alpha 1-Acid Glycoprotein present in samples reacts with the anti-Alpha 1-Acid Glycoprotein antibodies which have been adsorbed to the surface of polystyrene microtitre wells.  After the removal of unbound proteins by washing, anti-AGP antibodies conjugated with horseradish peroxidase (HRP), are added.  These enzyme-labeled antibodies form complexes with the previously bound AGP.  Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3,5,5-tetramethylbenzidine (TMB).  The quantity of bound enzyme varies directly with the concentration of AGP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of AGP in the test sample. Application: Method:ELISA Species: Storage: