Rat Kim-1 ELISA Kit 1 Kit (96 Wells) - 1 Kit

The principle of the double antibody sandwich ELISA is represented in Figure 1.  In this assay the KIM-1present in samples reacts with the anti-KIM-1antibodies which have been adsorbed to the surface of polystyrene microtitre wells.  After the removal of unbound proteins by washing, anti-KIM-1 antibodies conjugated with horseradish peroxidase (HRP), are added.  These enzyme-labeled antibodies form complexes with the previously bound  KIM-1.  Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3,5,5-tetramethylbenzidine (TMB).  The quantity of bound enzyme varies directly with the concentration of KIM-1 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of  KIM-1 in the test sample.  The quantity of  KIM-1 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Application: Method:ELISA Species: Storage: