Nickel Assay Kit - 100 assays

Nickel is one of four ferromagnetic elements (symbol Ni, at. Num. 28). Several enzymes depend on nickel for activity, including some ureases, carbon monoxide dehydrogenases (methane forming enzymes which reduce CO2 to CH4) and some hydrogenases which allow the production or removal of H2. Most of these activities are found in the archaebacteria. Nickel forms complexes with sulfhydryl compounds with significant absorbance in the UV/visible region in the presence of other ions. BioVisions Nickel Assay kit takes advantage of reaction of Ni+2 with mercaptoethanol in borate buffer to form a complex with strong absorbance bands from ~300 to 600 nm. Fe+2 and Co+2 interfere which necessitates an absorbance vs. wavelength ratio to determine correct Nickel concentration in mixed samples. Other ions tested (Mn+2, Cu+2, Zn+2) do not interfere with the assay. The assumption made is that no other ionic species are present in high enough concentration to interfere with the reaction. The assay is a simple method of quantitating Ni+2 in a variety of samples, which gives a linear range of 2 to 50 nmol Nickel containing less than 25 nmol Cobalt. Application: Method: Species: Storage:-20°C